Title: The modeling of the HIV Capsid with novel binding sites on Nucleoporin 153

Project Team: Paul Rowley, Shunji Li, Jagdish Patel

Start Date: August 2021

A molecular modeling approach will be used to assess whether homologs of NUP153 motif-1 interact with the HIV-1 capsid. We have preliminary binding data which suggest at least one of the motifs interacts with capsid. Physical binding measurement revealed that Motif-1 homologs, Motif-4, and Motif-5 had a measurable KD, suggesting that they directly bind capsid. What is unknown is if these peptides are interfacing with the same binding site as Motif-1.