Multidrug antibiotic persistence, which allows some cells that lack genetic resistance to survive antibiotic stresses by becoming dormant, is a major public health concern. This exploratory project will use data from state-of-the-art image cytometry and single-cell analysis in combination with mechanistic mathematical modeling to study the formaldehyde-sensing network that was recently discovered in Methylobacterium by the Marx Lab. The formaldehyde-sensing network in Methylobacterium shares many characteristics with antibiotic persistence, but has the advantage of allowing us to externally manipulate factors governing the transition from growth to stasis, and all the cells in a population go dormant. The research team wishes to develop mathematical models in combination with relevant experimentation 1) to study the ability the biochemical network to allow for distinct cell fate outcomes as a function of key parameters such as protein levels of EfgA 2) to analyze how stochasticity in the form of spontaneous fluctuations in protein levels, which can lead to a potentially toxic pulse of formaldehyde, influences cell transitions between phenotypes such as growth, death, or persistence. This pilot grant will position the researchers to explore fundamental processes associated with antimicrobial resistance, which if eventually manipulated could prevent disease and promote health.
Insights into Human Anamalous Visual Functions Using Molecular ModelingSeptember 1, 2019
Project Team: Jagdish Patel, Deborah Stenkamp, Dharmesh Patel, Jonathan Barnes Note: This pilot grant evolved out of the research produced by the working group, Evolution of Tandemly-Replicated Opsin Genes: Molecular Models that Predict Spectral Shifts. Vision is one of the most sophisticated biochemical system in humans and serves as a primary environmental input. In other organisms, […]
CMCI Team Wins Interdisciplinary AwardApril 26, 2019
The University Awards for Excellence recognize and encourage excellence in all forms at the academic level. Recently, the 2019 Interdisciplinary Award was presented to the Flow Ventilators Team for their collaborative efforts for working to improve treatments for asthma and other lung diseases. Team members include 4 researchers from 2 colleges: Tao Xing, associate professor […]
Determining the Role of Albumin Conformation in Enhanced Bone Repair and RegenerationAugust 28, 2018
Project Team: Matthew Bernards, Nathan Schiele, Dharmeshkumar Patel, Stephanie Haag In the US, over 130,000 surgical procedures each year require a bone graft material, but almost 30% of grafts fail. Recent studies have implicated albumin as important during bone repair and it has been adapted for improving scaffold integration with bone tissue. This is important […]